![]() ![]() These views highlight the difference in traces that result from the presence of a bead, and the incorporation process. ![]() This figure shows an example of output from a sensor on a 3‐series chip, with the upper panel being the raw voltages (mV units) sampled from the sensor during the course of 12 nucleotide flows, indicated by the labels on each bump, and the bottom trace shows that background subtracted signals, which makes clear 5 positive incorporation events occurring during these 12 flows. To isolated the incorporation signal explicitly, this background change in pH must be modeled and subtracted.Figure S7. Shown in blue is the trace from a flow in which single base incorporation occurred, whereas red shows a superimposed trace from another sensor in which no incorporation has occurred, and the ∼0.2 pH changes is due solely to pH changes in the solution flowing onto the chip. This figure shows the raw data from a 314 pHFET sensor, sampled at 100Hz and with the signal voltage (fundamental signal type) converted to pH units here for presentation. ![]() Typical raw signals from a 3‐series pHFET sensor. This illustrates the potential for achieving contiguous read lengths on 500‐base scale.Figure S6. ![]()
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